Human p53 Upregulated Modulator Of Apoptosis ELISA Kit from Innovative Research is intended for the quantitative determination of Human p53 Upregulated Modulator Of Apoptosis in biofluid samples, such as tissue homogenates, cell lysates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . PUMA present in samples reacts with an anti-Human PUMA capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-PUMA detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active PUMA in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Human PUMA. Detection Target: Human p53 Upregulated Modulator Of Apoptosis Reactivity: Human Range: 0.156-10ng/mL Sensitivity: 0.048ng/mL Storage Conditions: Store all components at 4┬░C This Human PUMA activity assay is for the quantitative determination of p53 Upregulated Modulator Of Apoptosis in samples including tissue homogenates, cell lysates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.